Table 1.
Experimental data for DHFR, including microbial source, growth conditions, stability, and factors influencing activity
| Microbe* | Tg† (°C) | Pg† (bar) | ΔGu† (kJ/mol) | kcat† (s−1) | Increase† | Res 27† |
Ref‡ |
|---|---|---|---|---|---|---|---|
| S. benthica (Sb) | 10 | 700 | 8.7±1.2 | 68.3±1.2 | Yes | D | [84] |
| S. benthica (Sb′) | 15 | 600 | 4.7±0.9 | 70.2±1.1 | No | D | [84] |
| S. violacea (Sv) | 10 | 300 | 8.0±0.5 | 86.8±2.0 | Yes | D | [84] |
| M. yayanosii (My) | 10 | 800 | NA | 80.3±2.0 | Yes | E | [83] |
| M. japonica (Mj) | 10 | 500 | NA | 109.2±1.1 | No | E | [83] |
| M. profunda (Mp) | 6 | 220 | 13.4 | 18.7±0.8 | Yes | E | [85]/[86] |
| P. profundum (Pp) | 15 | 280 | NA | 150.5±2.3 | No | D | [83] |
| S. frigidimarina (Sf) | 0 | 1 | 8.3±0.9 | 93.3±1.3 | No | D | [84] |
| S. oneidensis (So) | 30 | 1 | 6.7±1.0 | 45.1±1.1 | No | D | [84] |
| S. putrefaciens (Sp) | 37 | 1 | 8.3±1.4 | 98.7±1.7 | No | D | [84] |
| E. coli (Ec) | 37 | 1 | 26.9±3.7 | 19.2±0.5 | No | D | [84] |
| E. coli (Ec) | 37 | 1 | 24.7 | 16.4±3.6 | No | D | [85]/[86] |
| G. stearothermophilus (Gs) | 55 | 1 | NA | 14.6±1.3 | No | D | [85] |
| T. maritima (Tm) dimer | 80 | 1 | 144.4 | 0.10±0.01 | No | D | [85]/[86] |
*
Sb was isolated at 10,898 m; Sb′ was isolated at 6,356 m; G. is Geobacillus; T. is Thermatoga
†
Tg and Pg are from Ref. [63] for piezophiles; Res 27 is amino acid residue at position 27; Increase refers to relative activity with pressure; kcat of NADPH at 25 °C; ΔGu using urea at 15°C.
‡
Reference is for kcat at 25 °C and ΔGu at 15 °C unless two given, in which case, first is for kcat and second is for ΔGu