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. 2018 Jul 17;8:10780. doi: 10.1038/s41598-018-29236-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the supplementation with LOX/BMP1 supernatants on collagen deposition from fibroblast cultures. Collagen fractions as measured in Fig. 1 were analyzed in fibroblasts exposed to conditioned media from control or LOX- and BMP1-overexpressing cells and incubated with and without TGF-β1 for 4 days. (A) Soluble collagen in the supernatant. (B) Pepsin-solubilized collagen fraction associated to cell monolayer. (C) Insoluble collagen deposited into the matrix. (D) LOX-derived pyridinoline (PYD) cross-link levels in the deposited matrix from fibroblast cultures exposed to conditioned media as assessed by specific ELISA. Values are represented as μg collagen or concentration of PYD per million of cells (mean ± SEM, n = 6; *P < 0.05 vs the corresponding control values with TGF-β1).