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. 2018 Jul 17;3:28. doi: 10.1038/s41541-018-0064-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression analysis in CHO-Xpress® system of DBL1x-2× (3D7); 105 kDa, DBL1x-3× (3D7); 176 kDa, INT1-DBL3x (3D7); 123 kDa, DBL3x-4ε (FCR3); 86 kDa and DBL1x-6ε (3D7); 300 kDa. a Coomassie staining and b anti-His western blotting performed on crude culture supernatants after gel electrophoresis in reducing and non-reducing conditions. MW, molecular weight ladder. P, His-tagged protein used as a western blot positive control. All blots derive from the same experiment and were processed in parallel