Fig. 3.

Expression analysis in E. coli systems in different test conditions SHuffle express (1), BL21 (2, 4, 5, 6) and HMS174 (3) cells] of a DBL1x-2× (3D7), b DBL1x-3× (3D7), c ID1-DBL3x (3D7), and d DBL3x-4ε (FCR3). The left panels represent the Coomassie staining of crude culture supernatants after gel electrophoresis in reducing conditions at the time of induction (I) and at the time of harvesting (H). The right panels display the western blot analysis of protein production using an anti-His antibody. Detailed growth and induction parameters of each expression conditions are depicted in Table S2. Red arrows indicate the expected molecular weights of the proteins of interest. MW, molecular weight ladder. P: His-tagged protein used as a western blot positive control. All blots derive from the same experiment and were processed in parallel