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. 2018 Jul 17;3:28. doi: 10.1038/s41541-018-0064-6

Fig. 5.

Fig. 5

Immune recognition of erythrocytes infected by different parasite strains (NF54, FCR3, and 7G8) selected for different adhesive phenotypes (CSA and CD36) by vaccination-induced antibodies directed towards VAR2CSA-based constructs. Infected erythrocytes were incubated with rat sera samples diluted 1:50 in PBS 1% BSA. Erythrocyte-bound IgGs were detected using a rat IgG specific antibody PE-conjugated. Cells were then subjected to flow cytometry analysis. Results are expressed as the fold-increase in geometrical mean fluorescence intensity obtained with immune (Day 63) sera compared to non-immune sera. For each condition, the fold-increase values obtained for the diluted sera represent the pool of three rat sera samples