Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 17;9:2773. doi: 10.1038/s41467-018-05124-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — APC2 effect on global organization of microtubule polarity in dendrites. a Neurons transfected with RFP-tubulin, PA-GFP-tubulin and either control or APC2 shRNA on DIV11–12 were live imaged on DIV15–16 (schematic drawn by O.I.K.). b Representative images of control or APC2 depleted neuron with photoactivated region of dendrite undergoing microtubule bundle sliding. Red dotted line represents edges of photoactivated region at t = 0. Black dotted line represents edges photoactivated region at the corresponding time point. Time overlay has frame at t = 0 in magenta merged with t = 6 h in cyan. c Translocation distance of whole photoactivated region towards the cell body was measured (n = 21 dendrites control shRNA, n = 26 dendrites APC2 shRNA, N = 3, Two-Way ANOVA, Tukey post-hoc). d Distance of microtubule bundles sliding out of the photoactivated region both towards and away from cell body was measured (n = 8 dendrites control shRNA, n = 14 dendrites APC2 shRNA, N = 3, Two-Way ANOVA, Tukey post-hoc). e Schematic representation of microtubule photo-ablation in dendrites. f Neurons were transfected with GFP-MT + TIP and either control or APC2 shRNA at DIV11–12 and imaged DIV15–16. Proximal dendrites were photo-ablated 30 µm away from cell body and microtubule orientations were imaged for 3 min. Representative images of GFP-MT + TIP still frames, color-coded maximum projections and kymographs coded for direction of comets are shown. g Percentage + TIP comets in dendrites toward cell body post photo-ablation. (n = 14 dendrites control shRNA, n = 17 dendrites APC2 shRNA, p = 0.199, N = 2, Independent samples t-test). h Microtubule motor based super-resolution reconstruction of segments of proximal dendrites of neurons at DIV15 transfected with RFP-tubulin and either control or APC2 shRNA. Signal shown is derived from motor-PAINT. Red microtubules are minus-end-out, green are plus-end-out. Line scans are of green and red signals corresponding to microtubule orientations along 10 µm segments of dendrites. i Percentage mean red intensity to total was quantified as a measure of percentage minus-end-distal microtubules in the control or APC2 depleted neurons (n = 5 dendrites control shRNA, n = 7 dendrites APC2 shRNA, p = 0.588, N = 2, Independent samples t-test). Graphs represent mean ± SEM. *p ≤ 0.05. Scale bars represent 5 μm in b and 2 μm in h