Fig. 1.

Viral infection downregulates NDR1 expression in a type I IFN pathway-dependent manner. a Real-time PCR analysis of NDR1 mRNA in the peripheral blood from patients infected with RSV (n = 77) and healthy control (n = 40). b, c Real-time PCR (b) and immunoblot analysis (c) of NDR1 expression in peritoneal macrophages (PMs) infected with RSV, VSV, HSV, or MHV68. d, e Immunoblot analysis of NDR1 expression in PMs treated with IFNβ (100 IU ml⁻¹) (d), or with IFNα (100 IU ml⁻¹) (e). f, g Immunoblot analysis of NDR1 expression in lysates of IFNαR^+/+ and IFNαR^−/− (f) or STAT1^+/+ and STAT1^−/− (g) mouse immortalized bone marrow-derived macrophages infected with VSV. h, i ChIP-qPCR analysis of the histone modification in NDR1 promoter with H3K27me3 and H3K4me3 antibodies in lysates of Thp1 cells treated with VSV for 8 h (h) or with IFNα (100 IU ml⁻¹) for 4 h (i). Data are mean ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. *p < 0.05, **p < 0.01, ***p < 0.001. See also Supplementary Fig. 1