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. 2018 Jul 17;9:2648. doi: 10.1038/s41467-018-04868-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Genetic basis of IGF2BP1 for the enlarged body size of duck. a Spatial and temporal expression of IGF2BP1 in mallards and Pekin ducks measured by qPCR. b Immunofluorescence histochemistry staining for IGF2BP1 (green) in liver tissue of mallards and Pekin ducks at 1 day of age. Nuclei were stained with DAPI. c Fine mapping of the regulatory region of IGF2BP1. Blue, red, and orange bars (center panel) refer to mallard (M), Pekin (P), and heterozygotic (H) genotypes, respectively. R1-7 refer to seven recombinant types. The left and right box plots refer to body weight and IGF2BP1 expression levels, respectively. The numbers of individuals are given in brackets. The d feed intake, e feed efficiency, and f 3-week and 8-week serum glucose levels were measured in F₂ individuals with the mallard (M) and Pekin duck (P) genotypes. The indicated P values are based on one-way ANOVA. Box plots denote median (center line), 25–75th percentile (limits), minimum and maximum values without outliers (whiskers), and outliers (red and blue dots)