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. 2018 Jul 17;8:10804. doi: 10.1038/s41598-018-29151-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAP1 relocalises upon thrombin stimulation. (A) Platelets were allowed to spread on fibrinogen-coated coverslips and then stimulated with 0.1 U/mL thrombin for the indicated times, fixed with paraformaldehyde and immunostained with an anti-CAP1 antibody followed by an Alexa568-coupled secondary antibody (red) and counterstained with FITC-phalloidin for filamentous actin (green). Images were acquired with a fluorescence microscope equipped with a structured illumination attachment. Scale bar 5 µm. (B) Quantification of the pattern of CAP1 distribution upon thrombin stimulation. The proportions of cells with predominantly cortical or diffuse distribution of CAP1 in images like the ones shown in A were calculated from three independent experiments each performed in duplicate coverslips. At least 1000 cells per time point in each experiment were scored. Unclear refers to cells that were not sufficiently enough spread to make a judgment. Data are average ± SEM of four or five independent experiments. *p ≤ 0.05, **p ≤ 0.01, relative to 0 s, Mann-Whitney test.