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. 2018 Jul 17;8:10831. doi: 10.1038/s41598-018-29024-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Real-time profile of batch fermentation of E.coli BL21 (DE3) [pET41a + CHIKV nsP2pro] for expression of CHIKV nsP2pro. Culture was grown in Batch mode in 2XLB media with 1% glycerol and induced with 0.5 mM IPTG. Time profile for agitation (RPM), dissolved oxygen (%), temperature (°C) and pH; (b) OD (A₆₀₀); (c) Airflow rate (L/min). (d) Chromatogram of affinity chromatography for purification of CHIKV nsP2pro fusion protein (71 kDa). (e) SDS–PAGE analysis of purified nsP2pro (39 kDa) after tag removal. (f) Purified protein band was cut out, digested with trypsin and analysed further with mass spectrometry analysis. MALDI-TOF/MS spectrum of CHIKV nsP2pro protein tryptic digests in reflectron mode using HCCA as matrix.