. 2018 Jul 17;8:10755. doi: 10.1038/s41598-018-28916-7

Table 4.

List of prioritized protein targets by incorporating protein conservation among gut microbiomes (ranked according to equation (3)).

Rank	Locus number^	Gene	Product	Product size (aa)	Structural druggability*	Essential	Choke-point	Network centrality	Presence in pathogenic Kp (%)	Pathways involved^#	DrugBank Inhibitor^&
1	02296	argA	Amino-acid acetyltransferase	443	0.75	Yes	Yes	0.89	100	L-ornithine [B], L-arginine [B]	ND
2	01032	fabB	3-oxoacyl-[acyl-carrier-protein] synthase 1	407	0.74	Yes	Yes	0.64	100	Biotin [B], Fatty acids [B]	Approved (DB01034)
3	00796	pssA	CDP-diacylglycerol–serine O-phosphatidyltransferase	451	0.86	Yes	Yes	0.06	97	Phosphatidylethanolamine [B]	ND
4	01800	lpxD	UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase	341	0.82	Yes	Yes	0.07	100	Lipopolysaccharide [B]	ND
5	01909	murF	UDP-N-acetylmuramoyl-tripeptide–D-alanyl-D-alanine ligase	452	0.76	Yes	Yes	0.08	100	Peptidoglycan [B]	Experimental (DB06970)
6	01907	murD	UDP-N-acetylmuramoylalanine–D-glutamate ligase	438	0.71	Yes	Yes	0.02	100	Peptidoglycan [B]	Experimental (DB03801)
7	01797	lpxB	Lipid-A-disaccharide synthase	383	0.92	Yes	Yes	0.06	100	Lipopolysaccharide [B]	ND
8	05359	trpA	Tryptophan synthase alpha chain	270	0.63	Yes	Yes	0.52	100	L-tryptophan [B]	ND
9	00662	asd	Aspartate-semialdehyde dehydrogenase	368	0.58	Yes	Yes	0.05	100	L-lysine [B], L-threonine [B], L-methionine [B], L-homoserine [B]	Experimental (DB03502)
10	01899	lpxC	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase	306	0.78	Yes	Yes	0.29	100	Lipopolysaccharide [B]	Experimental (DB07861)
11	04194	lpxK	Tetraacyldisaccharide 4′-kinase	326	0.69	Yes	Yes	0	100	Lipopolysaccharide [B]	ND
12	01905	murG	undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase	356	0.8	Yes	Yes	0.06	100	Peptidoglycan [B]	Murgocil (ref.¹⁰⁶)
13	31828	murE	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate–2,6-diaminopimelate ligase	495	0.85	Yes	Yes	0.03	97	Peptidoglycan [B]	Experimental (DB03801)
14	05226	hpxT	5-hydroxyisourate hydrolase	109	0.6	Yes	Yes	0.007	100	Purine metabolism	ND
15	03831	dapE	Succinyl-diaminopimelate desuccinylase	375	0.81	Yes	Yes	0.08	100	L-lysine [B]	ND

^The K. pneumoniae Kp13 locus suffix ‘KP13_’ is omitted; ^*druggability of the protein considering the highest scoring pocket. ^#B: biosynthesis. ^&Data gathered from http://www.drugbank.ca for orthologs of each protein with relevance studied on a per-target basis. ND, not determined.