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. 2018 Jul 17;9:2783. doi: 10.1038/s41467-018-05266-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Loss of stromal PTEN decreases the DNA repair response in associated mammary epithelium. a Representative FACS plot defining CD24+CD29+ mammary epithelium (ErbB2;Fsp-cre;Pten^fl/fl). b Tumor-free survival of wild-type FVB/N mice injected orthotopically with ErbB2;Fsp-cre;Pten^fl/fl versus control ErbB2;Pten^fl/fl epithelium (n = 8/genotype). P value determined by Log-rank (Cox-Mantel). c Tumor volume (mean ± s.e.m.) at time of harvest after orthotopic injection of ErbB2;Fsp-cre;Pten^fl/fl (n = 8) versus control ErbB2;Pten^fl/fl (n = 7) epithelium. P value determined by Fisher’s exact. d Representative FACS plot defining ErbB2;Pten^fl/fl mammary epithelial subpopulations segregated by CD29 and CD24 (left: luminal and mammary stem cell (MaSC)) and CD61 (right: mature luminal and luminal progenitor). e Gene set enrichment analysis (GSEA) for DNA repair genes in ErbB2;Fsp-cre;Pten^fl/fl versus control ErbB2;Pten^fl/fl mature luminal epithelium. f Representative RAD51/keratin 8 dual immunofluorescence and quantification (mean ± s.e.m.) of epithelial cells isolated from ErbB2;Pten^fl/fl versus ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (3 Gy) in vitro and evaluated 6 h post-radiation. P value determined by Welch’s t-test (ErbB2;Pten^fl/fl n = 11 fields/3 mice, ErbB2;Fsp-cre;Pten^fl/fl n = 6 fields/2 mice). Scale bar = 20 μm. g Representative γ-H2AX immunofluorescence and quantification (mean ± s.e.m.) of epithelial cells isolated from ErbB2;Pten^fl/fl and ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (3 Gy) in vitro and evaluated 6 h post-radiation. P value determined by an unpaired, two-tailed Student’s t test (ErbB2;Pten^fl/fl n = 13 fields/3 mice, ErbB2;Fsp-cre;Pten^fl/fl n = 8 fields/3 mice). Scale bar = 20 μm