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. 2018 Jul 17;9:2783. doi: 10.1038/s41467-018-05266-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Loss of stromal PTEN increases genomic instability in associated epithelium. a Representative γ-H2AX immunohistochemistry and quantification (mean ± s.e.m.) of mammary epithelium in Pten^fl/fl, Fsp-cre;Pten^fl/fl, ErbB2;Pten^fl/fl and ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (6 Gy whole-body) and evaluated 6 h post-radiation. Both p values determined by unpaired, two-tailed Student’s t test (Pten^fl/fl n = 32 fields/3 mice, Fsp-cre;Pten^fl/fl n = 42 fields/3 mice, ErbB2;Pten^fl/fl n = 47 fields/5 mice, ErbB2;Fsp-cre;Pten^fl/fl n = 33 fields/3 mice). Scale bar = 20 μm. b Representative α-Tubulin/pericentrin dual immunofluorescence of epithelial cells isolated from ErbB2;Pten^fl/fl and ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (6 Gy whole-body) and evaluated 1 week post-radiation in vitro. Scale bar = 25 μm. c Representative metaphase spreads of epithelial cells isolated from ErbB2;Pten^fl/fl and ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (6 Gy whole-body) and evaluated 1 week post-radiation in vitro. Arrowhead = chromosome break. Scale bar = 10 μm. d Quantification of centrosome amplification (shown in b) and chromosomal aberrations (shown in c) in epithelial cells isolated from ErbB2;Pten^fl/fl and ErbB2;Fsp-cre;Pten^fl/fl mice irradiated (6 Gy whole-body) and evaluated 1 week post-radiation in vitro. Both p values determined by two-tailed, Fisher’s exact test (left: ErbB2;Pten^fl/fl n = 22 fields/2 mice, ErbB2;Fsp-cre;Pten^fl/fl n = 29 fields/2 mice; right: ErbB2;Pten^fl/fl n = 33 spreads/4 mice, ErbB2;Fsp-cre;Pten^fl/fl n = 55 spreads/4 mice)