Table 1.

PCR Primers used for deletion mapping^a.

Set	Sequence (5′-3′)	Tm (°C)
1	AGATTCTTGGGCGCTGTATC	55
1	GGTTGATCAGTTCGTTGCAATAG	55
2	CTCACCAGTGGCGTCAATAA	55
2	GCATACGTCAGCAAGGATCA	55
3	CGATCTCGGCGTCAAAGATT	55
3	GGTAGCGCTTGTTCAGGTATAG	55
4	TACCACCAGTGGGACTACAA	55
4	CAGGTTCGGGAAGATCGAAAG	55
5	CCACCGTTTGATGTTGGATTAG	62
5	GAAGTAGTTCGACCCACCTATG	62
6	CCTACACCGACAAAGACATCTAC	55
6	TGGCTACCTTCAGCTTGTTC	55
7	TGCGAAGGTCTCTCCTTTCTA	62
7	CCAAGCCCTCAGTTCGTTAAG	62
8	CGATATCGCAGTCAGGAGAGA	62
8	CTTCAGGCCATGGAAGACTATATG	62
9	CAGCGCGATCAATGCAATAG	62
9	GTCGCCAACATGCTCAATATC	62
10	TCCTGAACAGCACCATCATC	62
10	CAGTCATCGTCCACCCATTT	62
11	GATTCTGCAAATCTGTCTCGGTA	62
11	CTAGGGCAGTAAGTCGTTGATT	62

^aSet number refers to primer binding site locations shown in Figure 3A.