Figure S4.
Effects of PML loss or PML suppression on altered lipid metabolism in HBsAg-transgenic mice. In response to challenge with a high-fat (HF) diet, the phenotypic evolution (obesity and body fat mass accumulation) and pathological changes (the severity of steatosis and dysplasia) in the lipid metabolism of the body and liver were compared between HBsAgtg/0 mice with or without one or two copies of PML knockdown (n = 5-10 for each genotype). (A) Respective effects of PML loss and HBsAg accumulation. Squares represent in-situ zoomed regions. (B) Dose effects of PML and HBsAg. Foci of altered hyperplasia and dysplasia are indicated by arrows. Note that PML deficiency induces more steatosis and less dysplasia and that HBsAg stimulation causes more dysplasia and less steatosis. Foci of hyperplasia and dysplasia are indiced by arrows. Squares represent in-situ zoomed regions. (C) Synergistic effects of PML loss and HBsAg accumulation on weight changes in the body, fat and liver. Error bars indicate mean ± SD. Note that PML loss enhances the effects of HBsAg on dysplasia and steatosis and predisposes HBsAgtg/0 mice to body fat mass accumulation and obesity.