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. 2018 May 11;22(8):3740–3750. doi: 10.1111/jcmm.13590

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© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of MSC‐CM on LEC sprouting. LEC spheroids were embedded in collagen or fibrin gels and treated with VEGF‐C combined with bFGF and 100% MSC‐CM. PMA served as positive control, basal medium supplemented with 0.5% FCS as negative control. (A) Spheroids were grown in hanging drop culture. (B) Representative images of spheroids treated with different stimulations after 24 h. (C) Sprout length and number of spheroids treated with different stimulations (x‐axis) were quantified and displayed in bar graphs. (n = 2, duplicates, *P ≤ .05 compared to negative control)