FIGURE 1.

Each endogenous gene of CERK1 family in tomato was specifically silenced by its respective VIGS construct. (A) The relative transcript levels of SlLYK1, SlLYK12, and SlLYK13 in leaves infiltrated with VIGS-SlLYK1, -12, -13, or -GUS (control). (B) Relative transcript levels in roots. RNA was extracted from leaves and roots 4 weeks after leaf infiltration with Agrobacterium tumefaciens carrying the indicated constructs. Transcript levels were detected using qRT-PCR. Data are expressed as means ± SD from three biological replicates. Asterisks indicate significant differences from the VIGS-GUS control (Student’s t-test: ^∗P ≤ 0.05).