Figure 1.

Chronic ethanol feeding induces steatosis and apoptosis in the rat liver. A, H&E staining of the rat livers. Liver injury and steatosis were observed in ethanol‐fed rats. B, AST C, and ASLT expression levels were determined in the serum samples harvested from control‐ and ethanol‐fed rats. n = 8. D, Apoptosis was measured in the livers of control‐ and ethanol‐fed rats using the TUNEL assay. Green fluorescence indicates the positive signal of apoptosis. DAPI stain, blue fluorescence, was used to show nuclear location. n = 5. E, The expression of miR‐34a, Sirt1 and Met was determined in the livers of control‐ and ethanol‐fed rats. n = 7. F, Relative caspase 3 activities were determined in control and ethanol‐fed rat liver samples. n = 5~7. P‐value was calculated from the student t test. *P < .05; **P < .01, when compared to control