Figure 4.

MiR‐129 suppresses the proliferation of glioma stem cells. A, The expression of miR‐129 in glioma stem cells was significantly lower than that in the glioma tissue cells detected by qRT‐PCR. B, The expression of miR‐129 in miR‐129 mimics group dramatically increased, while that in miR‐129 inhibitor group significantly decreased observed by qRT‐PCR. C‐E, CCK‐8 assay and EdU assay showed that cell proliferation of glioma stem cells were significantly attenuated in miR‐129 overexpression group but remarkably enhanced in miR‐129 inhibitor group compared with NC group. F‐G, The sphere formation assay results also revealed that the sphere formation efficiency of glioma stem cells in miR‐129 mimics group was significantly lower than that in NC group, while the cells transfected with miR‐129 inhibitor presented higher sphere formation efficiency in comparison with NC group determined by sphere formation assay. Scale bar, 100 μm. **P < .01. ***P < .001, ****P < .0001, compared with NC group