Figure 4.

A, STAT3 was not significantly altered in the miRNA‐3127‐5p transduced A549 cells, and hEGF as an exogenous stimulus, 300 ng/mL for half an hour, there is still no change after hEGF stimulation. Immunofluorescence showed that STAT3 mainly distributed in the cytoplasm. B, Immunofluorescence showed that p‐STAT3 increased significantly in miRNA‐3127‐5p transduced A549 cells compared with knocked down and empty vector control. hEGF as an exogenous stimulus, 300 ng/mL for half an hour, the expression of pSTAT3 increased significantly after stimulation and mainly distributed in the nucleus