Figure 4.

Cardiac remodelling in post‐partum S10F‐Hsp20 females. Studies were performed in non‐transgenic (NTG) and S10F females following 3 pregnancies with age‐matched non‐pregnant females as controls. (A) Haematoxylin staining of ventricular sections. (B) Ratio of heart weight (g)/tibia length (cm) (n = 4‐5 hearts/group); (C) immunostaining of ventricular sections with fluorescence labelled wheat germ agglutinin; and (D) quantitative analysis of cardiomyocyte cross‐sectional area (n = 5 hearts/group). mRNA expression levels of (E) atrial natriuretic peptide (ANP) and (F) brain natriuretic peptide (BNP) after normalization to GAPDH (n = 3 hearts/group). Values represent mean ± SEM. *: P < .05; ** P < .01