Fig. 5. gO/CFs are retarding RF nucleation and growth. (A) Fit to ThT kinetics of hewL RFs below the COC with the analytical solution for nucleated polymerization (eqn (1)†). (B) Primary (δ) and secondary (κ) nucleation parameters obtained by the fits. (C, D) Typical ThT kinetics above the COC (blue circles), and their underlying RF kinetics (orange circles) obtained after subtracting the oligomeric portion of the fit (red line) from the data for hewL (C) and dimAβ (D). (E, F) RF lag times of hewL (E) and dimAβ (F) extracted from fits to experimental kinetics below (orange) and above (blue) the COC. The solid lines are power-law fits through these specific data sets below the COC (exponent α = –(0.17 ± 0.02) (hewL) and –(0.32 ± 0.06) (dimAβ)). Three or four, respectively, independent repeats of these measurements yielded power law values of α = –(0.24 ± 0.07) (hewL) and α = –(0.32 ± 0.05) (dimAβ).