Fig. 2. Kinetic characterization of pyrophosphorylation reactions. (a) Reaction of model phosphopeptide 14 with P-imidazolide 1. (b) Tabulated pseudo-first order kinetic data. Conditions: 100 mM P-imidazolide 1, 10 mM phosphopeptide 14, 267 mM ZnCl2. (c) Reaction progress kinetic data for the reaction of phosphopeptide 14 with reagent 1 in the presence of various additives. Data points represent averages from three replicate reactions (except for runs with added 2-mercaptoethanol, DTT, and guanidine HCl). Error bars are omitted for clarity but are depicted in Fig. S7.† Processed RPKA data is shown in Fig. S8.† Conditions: 10 mM peptide 4, 30 mM P-imidazolide 1, 80 mM ZnCl2, in 1 : 9 H2O : DMA at 45 °C.
