Fig. 3. (A) Summarized absolute fluorescence change (–ΔF, at λ = 520 nm) of the CHA–HCR system without H₁, H₂, H₄ or H₆ for 5 h. The black and red bars show the absolute fluorescence change of the CHA–HCR system without and with 50 nM initiator, respectively. Error bars were derived from n = 5 experiments. (B) Native polyacrylamide gel electrophoresis of the single hairpin H₃, H₄, H₅, H₆, H₁, or H₂, and the complex of H₃ + H₄ + H₅ + H₆ (i), H₃ + H₄ + H₅ + H₆ + T (ii), H₁ + H₂ (iii), H₁ + H₂ + I (iv), H₁ + H₂ + H₃ + H₄ + H₅ + H₆ (v), and H₁ + H₂ + H₃ + H₄ + H₅ + H₆ + I (vi). AFM images of the CHA–HCR mixture with (C) and without (D) initiator I. Inset: cross-section analysis of the resulting CHA–HCR nanowires.