Fig. 6.

VSV infection of human monocytes induces their differentiation into phenotypically immature dendritic cells. a Monocyte-enriched population isolated from fresh PBLs was mock-infected or VSV-infected with the MOI = 10. Flow cytometry analysis was performed to measure the expression of CD123 and CD11c markers. The percentage of VSV-G+ cells within total DC population (HLA-DR+CD14- cells) was assessed to prove the ongoing viral replication. The data from 1 representative sample are shown. b The results of phenotypic analysis of MDDCs after VSV infection (n = 4). Percentages of CD11c+ CD123- and CD11c+ CD123+ populations were calculated within HLA-DR+ population. Infection of VSV was measured in the total DC population (HLA-DR+CD14- VSV-G+). The average of 4 replicates (mean ± 95% confidence interval) is shown (t test, * p < 0.05). c Analysis of expression of CD40, CD83, and CD86 markers on the total DC population by flow cytometry (n = 4). Data from 1 representative sample are shown (solid line, mock-infected; filled histogram, VSV-infected).