Table 1.
Immunization records, PCR, culture, DNA sequencing and MAT results found in 33 dogs presenting clinical suspicion of leptospirosis treated at the Veterinary Hospital of University of São Paulo between 2013 and 2016
| Dog | PCR | DNA sequencing | Culture | Vaccination | MAT | MAT titration (serovar) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Blood | Urine | (< 1 year) | (> 100) | Can | Po | Cas | Co | Ic | Py | Br | Ha | Wo | Sh | Bu | Aus | Cyn | Aut | |||
| 1 | NP | (+) | L. interrogans | (−) | (+) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2 | (+) | (+) | L. interrogans | (−) | (−) | (+) | 400 | 200 | 200 | 100 | 200 | 200 | – | – | – | – | – | – | – | – |
| 3 | (−) | (+) | L. interrogans | (−) | (−) | (+) | – | 800 | – | 3200 | 3200 | – | 200 | – | – | – | – | – | – | – |
| 4 | (+) | (+) | L. interrogans | (−) | (−) | (+) | – | – | – | – | 100 | – | – | 800 | 800 | – | – | – | – | – |
| 5 | NP | (+) | L. interrogans | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 6 | (−) | (−) | (−) | (+) | (+) | – | – | – | – | – | – | – | – | – | 200 | – | – | – | – | |
| 7 | NP | (−) | (−) | (−) | (+) | – | – | – | – | – | – | – | – | – | – | – | – | 100 | – | |
| 8 | (−) | (+) | L. interrogans | (+) | (−) | (+) | – | 200 | – | 400 | 400 | – | – | – | – | – | – | – | – | – |
| 9 | (−) | (−) | (−) | (+) | (+) | 400 | – | – | 400 | 200 | – | 200 | – | – | – | 100 | 200 | – | – | |
| 10 | (+) | (−) | L. interrogans | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 11 | (−) | (−) | (−) | (−) | (+) | – | – | – | 800 | 400 | – | – | – | – | – | – | – | – | – | |
| 12 | (−) | (+) | L. interrogans | (−) | (+) | (+) | – | – | – | – | – | – | 400 | – | – | – | – | – | 400 | – |
| 13 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| a14 | (−) | (−) | (−) | (+) | (+) | – | 100 | – | – | – | – | – | – | – | – | – | – | – | 100 | |
| a15 | (−) | (+) | – | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 16 | (+) | (−) | L. interrogans | (−) | (−) | (+) | – | – | – | 400 | 200 | – | – | – | – | – | 200 | – | – | – |
| a17 | (+) | (+) | – | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 18 | (−) | (−) | (−) | (+) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| 19 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| 20 | (−) | (+) | – | (−) | (−) | (+) | – | – | – | 400 | 400 | – | – | – | – | – | – | 400 | – | – |
| 21 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| a22 | (−) | (+) | L. interrogans | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 23 | (−) | (−) | (−) | (+) | (+) | – | – | – | 200 | – | – | – | – | – | – | – | – | – | – | |
| a24 | (−) | (+) | L. interrogans | (+) | (+) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| a25 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| 26 | (+) | (+) | – | (−) | (+) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 27 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| 28 | (−) | (−) | (−) | (−) | (−) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| 29 | (−) | (−) | (−) | (−) | (+) | – | – | 100 | 800 | 400 | – | – | – | – | 400 | – | 400 | – | – | |
| a30 | (−) | (−) | (−) | (−) | (+) | – | – | – | 200 | 200 | – | – | – | – | – | – | – | – | – | |
| 31 | (−) | (+) | L. interrogans | (−) | (−) | (+) | – | – | 800 | – | – | – | – | – | – | – | – | – | – | – |
| 32 | (+) | (+) | L. interrogans | (−) | (−) | (+) | – | – | – | 1600 | – | – | – | – | – | – | 200 | 800 | – | – |
| 33 | (+) | (+) | L. interrogans | (−) | (−) | (+) | – | – | – | 200 | 100 | – | – | – | – | – | 100 | – | – | – |
a Dogs included in the prospective evaluation
NP not performed, Can Canicola, Po Pomona, Cas Castellonis, Co Copenhageni, Ic Icterohaemorrhagiae, Py Pyrogenes, Br Bratislava, Ha Hardjo, Wo Wollfii, Sh Shermani, Bu Butembo, Aus Australis, Cyn Cynopteri, Aut Autumnalis