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. 2018 Jul 17;19:542. doi: 10.1186/s12864-018-4937-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterise of evolutionary forces in H. manillensis. a ENC-GC3 plot. The solid line represents the expected curve when codon usage bias is only affected by mutation pressure. b Frequency distribution of ENC. c PR2-bias plot. Using the values of A3/(A3 + U3) against G3/(G3 + C3). d Neutrality plot analysis of GC12 and GC3 contents