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. 2018 Jul 17;11:95. doi: 10.1186/s13045-018-0638-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The role of Ubc13 in Ube2v1 mediates Sirt1 ubiquitination in CRC cells. a Immunoprecipitation assay for the exogenous interaction between Ube2v1 and Sirt1in SW480 cells transfected with Flag-Ube2v1 and Myc-Sirt1. b Immunoprecipitation assay for the exogenous and endogenous interaction between Ubc13 and Sirt1 in SW480 cells. c Ubiquitination assays of endogenousSirt1 in the lysates from SW480 cells cotransfected with GFP-Ube2v1, HA-Ub, two siRNAs targeting Ubc13, or vector control. The cells were treated with or without MG132 (20 μM) before harvest and then immunoprecipitated them with anti-Sirt1 antibody. d Ubiquitination assays of endogenous Sirt1 in the lysates from SW480 cells cotransfected with GFP-Ube2v1, HA-Ub, NSC697923 (a small-molecule inhibitor targeting Ubc13), or DMSO control. The cells were treated with or without MG132 (20 μM) before harvest and then immunoprecipitated them with anti-Sirt1 antibody