Fig. 5.
Effects of rapamycin on Ube2v1-mediated lung metastasis in the orthotropic mouse xenograft models of CRC. a Lung index and numbers of metastatic lung nodules of mice administrated intravenously with SW480 cells stably overexpressing Ube2v1 (2 × 106 cells) were determined (n = 6 per group). b Lung index and numbers of metastatic lung nodules of mice administrated intravenously with SW480 cells stably knocked down expression of Ube2v1 (2 × 106 cells) were determined (n = 6 per group). Representative photographs of the lung with metastatic nodules are shown (arrow heads). Representative micrographs of the lung with metastatic cells are shown by H&E staining at a magnification of × 200. M metastatic lesion, N adjacent normal lung tissue. Expressions of E-cadherin and LC3-II were detected after Ube2v1 was overexpressed with or without rapamycin (final concentration 10 ng/ml) stimulation. c Representative photographs of the lung with metastatic nodules are shown. d Representative micrographs of the lung with metastatic cells are shown by H&E staining at a magnification of × 100 (left) and × 200 (right). e Lung index and numbers of metastatic lung nodules of mice were determined (n = 6 per group). M metastatic lesion, N adjacent normal lung tissue. Error bars represent mean ± s.d. Statistical significance was determined by a two-tailed, unpaired Student’s t test. *p < 0.05, **p < 0.01. f Expressions of Ube2v1, Beclin1, and E-cadherin were analyzed by immunohistochemical staining. Error bars represent mean ± s.d. Statistical significance was determined by a two-tailed, unpaired Student’s t test. *p < 0.05