Fig. 2.

Analysing printed DNA reproducibility. a Analysis of expression library quality by enzymatic digestion. The BsrGI enzyme was used for digestion; two DNA fragments were produced. The 4 kbp band corresponds to the linearized vector, whilst the smaller fragment corresponds to the released insert. The presence of more than one band below 4 kbp indicate that the insert contains restriction sites for BsrGI. MWM: molecular weight marker. At least two fragments were observed in all cases. b Scanning images showing the spots (i.e. DNA printed onto cell surface before expression). The Figure shows 16 sub-arrays (containing 29 clones and negative controls) for each array. Each region is represented by two sub-arrays. c Inter-slide reproducibility showing the relationship between normalized PicoGreen signals for clones printed in different arrays. d Intra-slide reproducibility showing the relationship between clones printed on different sub-arrays