Table 3.
Specific primers used to amplify the transcripts of NK homeobox genes from cDNA of the onychophoran E. rowelli
| Gene | Fragment length (in bases) | Direction | Primer sequence | Annealing temperature (°C) |
|---|---|---|---|---|
| NK1 | 730 | Forward | ATGTTGGTGTATTGCCGGAGG | 62 |
| Reverse | GTAGGCAAACTTGTGACTGGG | |||
| NK3 | 649 | Forward | TCTAAGCGACACTCTCACAGC | 62 |
| Reverse | AGGAAGGATACATGACAGGCC | |||
| NK4 | 900 | Forward | TAAAGATATTTTAAATCTTAGCGAACAAG | 58 |
| Reverse | GCCACATATTATTTACACAGTGACATC | |||
| NK5 | 611 | Forward | CCATCGACTCAAGTTCCAACG | 62 |
| Reverse | ATGTTTGCTGCCTCCATCTCG | |||
| NK6 | 656 | Forward | GACTACACCAACCCAAACACG | 62 |
| Reverse | TGGAACCATACCTTGACCTGG | |||
| Msx | 712 | Forward | TTGCAGGCGAGTAGTAACTCG | 62 |
| Reverse | ATGACAGTCCTAAAGCAGGCG | |||
| Lbx | 579 | Forward | CGAAGAGCATTTGAAAGCGC | 58 |
| Reverse | GTCACGTGTATTCGTGTAGC | |||
| Tlx* | 703 | Forward | GCTAGAGACAATTCTCC | 43 |
| Reverse | GAACTGTCCTCAGCCC | |||
| NK2.2 | 486 | Forward | ATGGCTCTAAATGGGTCAAAAAG | 62 |
| Reverse | TGGTGGATCTCCTGCATTTG |
*Primer sequences obtained from Ref. [129]