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. 2018 Apr 10;115(2):251–262. doi: 10.1016/j.bpj.2018.03.016

Figure 2.

Figure 2

Engineered strain ΔinlCPhyper-inlC-GFP11 displays normal in vitro growth, protein secretion, and infectivity. (a) The growth curve of Listeria strains at 30°C is shown. Errors bars indicate STD from n = 3 experiments. (b) An immunoblot of Listeria cell lysates after 25 h growth using a polyclonal anti-InlC antibody is shown. (c) A Coomassie-stained SDS-PAGE gel of secreted proteome of wt, ΔinlC, and ΔinlCPhyper-inlC-GFP11 strains collected by trichloroacetic acid precipitation of supernatants from Listeria cultures after 25 h growth is shown.