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. 2018 Apr 10;115(2):251–262. doi: 10.1016/j.bpj.2018.03.016

Figure 6.

Figure 6

High throughout quantification of GFP complementation during Listeria infection. (a) HeLa cells were infected with ΔinlCPhyper-inlC-GFP11 and ΔinlCpInlC-inlC-GFP11 side by side, and infections were visualized over time in parallel in the same experimental setup. A representative infection (ΔinlCpInlC-inlC-GFP11) at 3 h postinfection (left panel) vs. 6.5 h postinfection (right panel) is shown. The inset indicates examples of Listeria cells in the DIC channel. Scale bars, 20 μm. Images in the GFP channel are presented at the same intensity levels. (b) Quantification of GFP fluorescence intensity for the time course shown in (a) is given. The arrows indicate 3 and 6.5 h postinfection. (c) The mean GFP fluorescence signal for all cells in the experiment was determined at 3 h postinfection vs. 6.5 h postinfection (one experiment; n = 128 cells for ΔinlCPhyper-inlC-GFP11 and n = 75 cells for ΔinlCpInlC-inlC-GFP11). Statistical significance was determined using a Tukey honest significant difference ANOVA test. To see this figure in color, go online.