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BMDMs expressing GFP1-10 and the nuclear TagBFP infected with ΔinlCpInlC-inlC-GFP11. GFP complementation signal increases over the course of infection for infected cells (a) but not for uninfected cells (b). The fluorescence signal was adjusted to have the same thresholds for (a) and (b). GFP complementation may accumulate in nontransfected cells as macrophages consume other transfected and infected cells. Scale bars, 20 μm. To see this figure in color, go online.
