Figure 6.

Structural weakening of TCTN2^−/− cilia with a large bending curvature and a large tapering angle. (A) Super-resolved images of ciliary marker GT335 reveal two ciliary boundaries in detail for curvature calculation and subsequent flexural rigidity estimation. (B) Analysis of the tapering angle of two ciliary boundaries by computationally straightening the ciliary images in (A) shows diameter shrinkage of axonemal configuration toward the ciliary tip in TCTN2^−/− cells. (C and D) Statistical analysis of the maximal curvature and tapering angle of GT335 pattern for the data set in (A) and (B) are shown (^∗∗p < 0.01 with unpaired t-test). To see this figure in color, go online.