Table 1.

Midpoint and Onset Temperatures of Structural Transition at Distinct Hierarchical Structure Levels

	A Tertiary Structure q = 0.05–0.2 Å−1	B Internal Structure q = 0.25–0.8 Å−1	C Oligomerization q = 0.07–0.09 Å−1	D Sheet Stacking q = 0.55–0.65 Å−1	E Cross-β Structure q = 1.3–1.38 Å−1	F Cross-β Structure
By Sight
Tm (gly = 0% v/v)	79.0 ± 0.5°C	74.8 ± 0.5°C	73.2 ± 0.5°C	76.2 ± 0.5°C	75.8 ± 0.5°C	–
Tm (gly = 10% v/v)	79.4 ± 0.5°C	76.9 ± 0.5°C	80.3 ± 0.5°C	78.3 ± 0.5°C	76.2 ± 0.5°C	–
Tm (gly = 20% v/v)	82.2 ± 0.5°C	78.8 ± 0.5°C	80.0 ± 0.5°C	80.2 ± 0.5°C	80.2 ± 0.5°C	–
Ton (gly = 0% v/v)	–	–	–	–	–	73.8 ± 1.3°C
Ton (gly = 10% v/v)	–	–	–	–	–	76.3 ± 1.3°C
Ton (gly = 20% v/v)	–	–	–	–	–	77.5 ± 2.5°C
By Sigmoidal Fit
Tm (gly = 0% v/v)	79.3 ± 0.1°C	75.6 ± 0.4°C	70.4 ± 1.5°C	77.3 ± 0.5°C	78.1 ± 1.6°C	–
Tm (gly = 10% v/v)	80.0 ± 0.3°C	77.7 ± 0.3°C	79.9 ± 0.6°C	79.2 ± 0.3°C	79.2 ± 1.2°C	–
Tm (gly = 20% v/v)	81.0 ± 2.3°C	79.5 ± 0.4°C	79.3 ± 0.3°C	79.9 ± 0.4°C	80.0 ± 2.1°C	–

Summary of transition midpoint temperature, T_m, at different hierarchal structure levels. Columns A–E show T_m values obtained from Fig. 16, and column F shows T_on values estimated from Fig. 14. T_m values were determined both by sight and by sigmoid function fitting.