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TNFRSF12A is a novel target of miR-149. (a) The miR-149 putative binding sites and the corresponding mutant sites of TNFRSF12A. (b), (c) Expression level of TNFRSF12A was examined by qRT-PCR and Western blot analysis in the miR-149 and miR-NC groups. (d) Luciferase activity of TNFRSF12A 3’-UTR was detected after miR-149 vector transfection. Data were normalized to the luciferase activity after transfection with miR-NC. Data are the means ± SEM of three experiments.
**P < 0.01.
