Figure 5.

Regulatory T cell (Treg) trafficking is not impaired by absence of IL-10 receptor a (IL-10Ra) on Tregs. Analyses in A–E derive from NTN-injected mice at day 8 (n=16 versus n=18). (A) Representative immunohistochemical staining and (B) quantification of Foxp3⁺ renal Tregs. (C) Quantification of the renal Foxp3-to-CD3 ratio as counted from immunohistochemically stained sections. (D) Representative FACS plot and quantification of renal Foxp3⁺ Treg infiltration. (E) FACS analyses of renal Tregs expressing the Treg17-characteristic chemokine receptor CCR6 or the Treg1 hallmark chemokine receptor CXCR3. (F) FACS analyses of Treg percentages in spleens and renal lymph nodes (LNs) at day 3 after NTN. (G) FACS analyses of CCR7 expression on Tregs in the indicated organs at day 3 after NTN; n=8 mice per group were analyzed in F and G. Circles represent individual animals, and horizontal lines show mean values. *P<0.05.