Figure 3. Complete Freund's adjuvant (CFA) activated NLRP3 inflammasome signaling in mice with trigeminal neuropathic pain. Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and interleukin-18 (IL-18) were assayed using a double antibody sandwich ELISA at each time-point (A). The mRNA expression of NLRP3, IL-1β, and IL-18 were measured by RT-qPCR at each time-point (B) (n=6). Data are reported as means±SD. *P<0.05 vs control group (NC) (ANOVA followed by a post hoc Tukey’s test).
