Figure 6. Schematic representation of the putative miR-186 binding site in the 3′UTR of NLRP3 predicted by the miRanda Target database (A). The ND8/34 cells were co-transfected with the wild and mutant (Mut) of NLRP3-3′-UTR and miR-186 mimics, inhibitors, or scramble, and the luciferase activity assay was performed after 48 h transfection (B). The ND8/34 cells were transfected with miR-186 mimics, inhibitors or scramble, and the mRNA (C) and protein (D) expressions of NLRP3 were measured by RT-qPCR and western blotting, respectively (n=6 at each time-point). Data are reported as means±SD. *P<0.05 vs control group (NC) (ANOVA followed by a post hoc Tukey’s test).