Figure 7. Over-expressed miR-186 alleviated trigeminal neuropathic pain by inhibiting NLRP3 inflammasome signaling. In complete Freund's adjuvant (CFA)-injected mice with miR-186 mimics treatment, the head withdrawal thresholds were measured in ipsilateral orofacial regions (A). The mRNA expressions of NLRP3, IL-1β, and IL-18 were measured by RT-qPCR assay (B). The protein expressions of NLRP3, IL-1β, and IL-18 were measured by western blotting (C, D). Data are reported as means±SD for n=6 in each group. *P<0.05 vs control group (NC); ^#P<0.05 vs CFA-injected group (ANOVA followed by a post hoc Tukey’s test).