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. 2018 Jul 10;9(4):e01136-18. doi: 10.1128/mBio.01136-18

TABLE 1 .

Identification of selected proteins from 2-D DIGE of B. burgdorferi (pH 3 to 10)a

Spot no. PER, expt 1/expt 2 Top-ranked protein (species) Accession no. Protein MW Protein pI
5 −2.75/−3.77 Integral OMP p66 (B. burgdorferi B31) gi|15594948 68,130 6.04
6 −3.02/−3.64 Membrane protein (B. burgdorferi) gi|700323990 68,130 6.04
7 −2.94/−3.41 Membrane protein (B. burgdorferi) gi|700323990 68,130 6.04
8 −1.45/−2.05 Integral OMP p66 (B. burgdorferi B31) gi|15594948 68,130 6.04
9 −1.68/−2.06 ABC TSBP (B. burgdorferi) gi|700323739 62,329 9.16
13 −1.55/1.27 Oligoendopeptidase F (B. burgdorferi) gi|499192176 69,679 6.84
15 −1.23/−2.79 ABC TSBP (B. burgdorferi) gi|700323739 62,329 9.16
16 −2.08/−1.95 Extracellular SBP (B. burgdorferi) gi|482681275 62,319 9.12
19 −2.80/−3.07 Glycerol kinase gi|6685462 55,571 7.02
20 −2.74/−3.53 Glycerol kinase gi|6685462 55,571 7.02
21 −1.96/−2.33 Glycerol kinase gi|6685462 55,571 7.02
24 −1.74/−2.42 Glycerol kinase gi|6685462 55,571 7.02
25 −2.63/−3.08 GPDH (B. burgdorferi) gi|480313620 54,478 8.89
26 −2.51/−2.89 GPDH (B. burgdorferi) gi|480313658 55,223 8.88
38 −2.33/−1.31 GAPDH (B. burgdorferi) gi|146743622 34,965 6.79
39 −2.17/−1.13 GAPDH gi|3915702 36,232 7.74
46 −1.74/−1.77 Chain E, Lyme disease antigen OspA gi|11514691 27,623 8.35
48 −1.83/−1.87 Putative lipoprotein (B. burgdorferi ZS7) gi|218164577 36,439 8.88
51 −2.20/−2.56 Chain E, Lyme disease antigen OspA gi|11514691 27,623 8.35
73 −1.99/−4.76 Peptide deformylase (B. burgdorferi) gi|488738649 19,104 6.32
75 −1.53/−1.92 NDPK (B. burgdorferi) gi|488620324 19,337 6.52
82 −1.83/−2.89 Integral OMP p66 (B. burgdorferi B31) gi|15594948 68,130 6.04
83 −2.42/−3.27 Integral OMP p66 (B. burgdorferi B31) gi|15594948 68,130 6.04
85 −2.43/−3.64 Integral OMP, partial (B. burgdorferi) gi|697996210 26,233 6.04
86 −2.14/−2.13 Chain E, Lyme disease antigen OspA gi|11514691 27,623 8.35
87 −2.81/−4.11 Antigen, p83/100 (B. burgdorferi Bol26) gi|226233237 79,324 5.09
89 −1.99/−2.60 P41, partial (B. burgdorferi) gi|483603 35,730 5.54
a

Abbreviations: PER, protein expression ratio; OMP, outer membrane protein; TSBP, transporter substrate-binding protein; SBP, solute-binding protein; GPDH, glycerol 3-phosphate dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NDPK, nucleoside-diphosphate kinase.