TABLE 1 .
Spot no. | PER, expt 1/expt 2 | Top-ranked protein (species) | Accession no. | Protein MW | Protein pI |
---|---|---|---|---|---|
5 | −2.75/−3.77 | Integral OMP p66 (B. burgdorferi B31) | gi|15594948 | 68,130 | 6.04 |
6 | −3.02/−3.64 | Membrane protein (B. burgdorferi) | gi|700323990 | 68,130 | 6.04 |
7 | −2.94/−3.41 | Membrane protein (B. burgdorferi) | gi|700323990 | 68,130 | 6.04 |
8 | −1.45/−2.05 | Integral OMP p66 (B. burgdorferi B31) | gi|15594948 | 68,130 | 6.04 |
9 | −1.68/−2.06 | ABC TSBP (B. burgdorferi) | gi|700323739 | 62,329 | 9.16 |
13 | −1.55/1.27 | Oligoendopeptidase F (B. burgdorferi) | gi|499192176 | 69,679 | 6.84 |
15 | −1.23/−2.79 | ABC TSBP (B. burgdorferi) | gi|700323739 | 62,329 | 9.16 |
16 | −2.08/−1.95 | Extracellular SBP (B. burgdorferi) | gi|482681275 | 62,319 | 9.12 |
19 | −2.80/−3.07 | Glycerol kinase | gi|6685462 | 55,571 | 7.02 |
20 | −2.74/−3.53 | Glycerol kinase | gi|6685462 | 55,571 | 7.02 |
21 | −1.96/−2.33 | Glycerol kinase | gi|6685462 | 55,571 | 7.02 |
24 | −1.74/−2.42 | Glycerol kinase | gi|6685462 | 55,571 | 7.02 |
25 | −2.63/−3.08 | GPDH (B. burgdorferi) | gi|480313620 | 54,478 | 8.89 |
26 | −2.51/−2.89 | GPDH (B. burgdorferi) | gi|480313658 | 55,223 | 8.88 |
38 | −2.33/−1.31 | GAPDH (B. burgdorferi) | gi|146743622 | 34,965 | 6.79 |
39 | −2.17/−1.13 | GAPDH | gi|3915702 | 36,232 | 7.74 |
46 | −1.74/−1.77 | Chain E, Lyme disease antigen OspA | gi|11514691 | 27,623 | 8.35 |
48 | −1.83/−1.87 | Putative lipoprotein (B. burgdorferi ZS7) | gi|218164577 | 36,439 | 8.88 |
51 | −2.20/−2.56 | Chain E, Lyme disease antigen OspA | gi|11514691 | 27,623 | 8.35 |
73 | −1.99/−4.76 | Peptide deformylase (B. burgdorferi) | gi|488738649 | 19,104 | 6.32 |
75 | −1.53/−1.92 | NDPK (B. burgdorferi) | gi|488620324 | 19,337 | 6.52 |
82 | −1.83/−2.89 | Integral OMP p66 (B. burgdorferi B31) | gi|15594948 | 68,130 | 6.04 |
83 | −2.42/−3.27 | Integral OMP p66 (B. burgdorferi B31) | gi|15594948 | 68,130 | 6.04 |
85 | −2.43/−3.64 | Integral OMP, partial (B. burgdorferi) | gi|697996210 | 26,233 | 6.04 |
86 | −2.14/−2.13 | Chain E, Lyme disease antigen OspA | gi|11514691 | 27,623 | 8.35 |
87 | −2.81/−4.11 | Antigen, p83/100 (B. burgdorferi Bol26) | gi|226233237 | 79,324 | 5.09 |
89 | −1.99/−2.60 | P41, partial (B. burgdorferi) | gi|483603 | 35,730 | 5.54 |
Abbreviations: PER, protein expression ratio; OMP, outer membrane protein; TSBP, transporter substrate-binding protein; SBP, solute-binding protein; GPDH, glycerol 3-phosphate dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NDPK, nucleoside-diphosphate kinase.