Phylogenetic affiliation of “Candidatus Nitrotoga fabula.” The consensus tree, which is based on 16S rRNA gene sequences of cultured and uncultured members of the candidate genus “Nitrotoga” shows the position of the “Ca. Nitrotoga fabula” isolate (indicated in orange) in this genus. Other cultured (enriched) “Ca. Nitrotoga” strains are indicated by boldface type. Pie charts indicate statistical support of branches based on maximum likelihood (RAxML; 1,000 bootstrap iterations) and neighbor joining (NJ) (1,000 bootstrap iterations). For PhyML, no bootstrap analysis was performed and gray indicates the presence of a branch. Bar, 0.01 estimated substitutions per nucleotide.