FIG 2 .

25-Hydroxycholesterol (25HC) is not required for LXR expression and suppresses MHV68 replication in an LXR-independent manner. (A) Primary macrophages derived from BL6 and CH25H^−/− mice were infected at an MOI of 5 for 24 h. LXRα and LXRβ expression was measured by qRT-PCR. (B) Primary macrophages derived from BL6 and LXR^−/− mice were infected at an MOI of 0.01 and then immediately treated with 2 µM 25HC or vehicle control for 72 h. Data shown are representative results from at least two independent experiments.