FIG 7 .

Activity of fatty acid and cholesterol synthesis pathways is increased and antiviral effects of fatty acid synthesis inhibition are attenuated in LXR^−/− macrophages. (A to C) BMDM of the indicated genotypes were infected at an MOI of 5 or mock infected. Macrophages were exposed to 5 µCi [³H]acetic acid for 8 h (16 to 24 h postinfection). At 24 h postinfection, macrophages were harvested; cholesterol (A), fatty acids (B), and triglycerides (C) data were resolved by TLC; and incorporation of ³H label was quantified by scintillation counting. DPM, disintegration per minute. (D and E) BMDM of indicated genotypes were infected at an MOI of 0.01 and treated with 10 µg/ml TOFA or vehicle control. Viral titers were measured at the indicated times postinfection. Data are representative of results from at least two independent experiments.