TABLE 2.
Primers used in this study
| Primer name | Sequencea | Use |
|---|---|---|
| Thio-1 | ACGCGGATCCATGTCTGCGACTATTGTA | Recombinant protein |
| Thio-2 | ACGCGTCGACTTAAACGTTTTCGTCAAT | Recombinant protein |
| Up_Fw | GCGTCGACCCCATATTCACCATAATCTG | SOE-PCR |
| Up_Rv | GGATCCACTACTGGCGCGGATCCGGTACGGCTCCAATTTTTAG | SOE-PCR |
| Dn_Fw | GGATCCGCGCCAGTAGTGGATCCGACGAAAATGTTTAAG | SOE-PCR |
| Dn_Rv | GCGTCGACTCTTCTGGGCGCTCATC | SOE-PCR |
| TrxA_Fw | CTAAAAATTGGAGCCGTACC | Screening |
| TrxA_Rv | CGTATTTTCATCTGTAACGTTACG | Screening |
| ABC_Fw | GTCGTAACAAGGTAGCCGTA | Strain typing |
| ABC_Rv | GGTGGGTTCCCCCATTCAGA | Strain typing |
| pIgRKO_FW | GAACTCTTGTCTTTTGTCTCC | pIgR genotyping |
| pIgRKO_RV | TCCAGACTGCCTTGGGAAA | pIgR genotyping |
| pIgRWT_FW | GAACTCTTGTCTTTTGTCTCC | pIgR genotyping |
| pIgRWT_RV | CTCGCCTGAATACTCCTTG | pIgR genotyping |
a
Boldface indicates restriction enzyme cleavage sites. Underlining indicates complementary sequence.