TABLE 1 .
P. aeruginosa strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| PAKa | Wild type | 52 |
| AJDP566 | pscC::pAJD1 | 18 |
| AJDP730 | ΔctpA | 18 |
| AJDP1091 | ΔlbcA::aacC1 | This study |
| AJDP1133 | Φ[ctpA-FLAG-His6] | This study |
| AJDP1140 | ctpA-S302A | This study |
| AJDP1153 | Φ[ctpA-S302A-FLAG-His6] | This study |
| AJDP1228 | ΔmepM | This study |
| AJDP1229 | ΔctpA ΔmepM | This study |
| AJDP1230 | ΔlbcA::aacC1 ΔmepM | This study |
| Plasmids | ||
| pHERD20T | Ampr pMB1 ori araBp expression vector | 53 |
| pET-24b(+) | Kanr pMB1 ori T7p expression vector | Novagen |
| pEX18Ap | Ampr pMB1 ori oriT sacB+ | 54 |
| pVLT35 | Smr Spr RSF1010 ori tacp expression vector | 55 |
| p34s-Gm | Apr aacC1+ (Gmr) pUC ori | 56 |
| pAJD2290 | T7p-′ctpA-His6 in pET-24b(+) | 18 |
| pAJD2003 | T7p-′oprF-His6 in pET-24b(+) | This study |
| pAJD2350 | tacp-ctpA-His6 in pVLT35 | 18 |
| pAJD2351 | tacp-ctpA-S302A-His6 in pVLT35 | 18 |
| pAJD2226 | araBp-ctpA-His6 in pHERD20T | 18 |
| pAJD2227 | araBp-ctpA-S302A-His6 in pHERD20T | 18 |
| pAJD2443 | tacp-lbcA-FLAG in pVLT35 | This study |
| pAJD2653 | T7p-′lbcA-His6 in pET-24b(+) | This study |
| pAJD2655 | T7p-′ctpA-S302A-His6 in pET-24b(+) | This study |
| pAJD2779 | araBp-mepM-FLAG in pHERD20T | This study |
| pAJD2801 | araBp-PA3623-FLAG in pHERD20T | This study |
| pAJD2809 | T7p-′mepM-His6 in pET-24b(+) | This study |
| pAJD2816 | araBp-PA1812-FLAG in pHERD20T | This study |
| pAJD2817 | araBp-PA4404-FLAG in pHERD20T | This study |
| pAJD2818 | araBp-PA3787-FLAG in pHERD20T | This study |
| pAJD2820 | araBp-PA1198-FLAG in pHERD20T | This study |
| pAJD2821 | araBp-PA1199-FLAG in pHERD20T | This study |
| pAJD2832 | T7p-′PA1198-His6 in pET-24b(+) | This study |
| pAJD2833 | T7p-′PA3787-His6 in pET-24b(+) | This study |
| pAJD2834 | T7p-′PA4404-His6 in pET-24b(+) | This study |
| pAJD2835 | araBp-PA0639-FLAG in pHERD20T | This study |
| pAJD2836 | araBp-PA1844-FLAG in pHERD20T | This study |
| pAJD2837 | araBp-PA2272-FLAG in pHERD20T | This study |
| pAJD2839 | araBp-PA5363-FLAG in pHERD20T | This study |
| pAJD2840 | araBp-PA5551-FLAG in pHERD20T | This study |
| pAJD2842 | araBp-PA4418-FLAG in pHERD20T | This study |
| pAJD2844 | T7p-′PA1199-His6 in pET-24b(+) | This study |
| pAJD2877 | araBp-rlpA-FLAG in pHERD20T | This study |
a
All P. aeruginosa strains shown are derivatives of strain PAK.