TABLE 2 .

Commercial kits and immunologic reagents tested in the ferret model

Product type and namea	Specificity	Clone	Isotype	Host	Vendor	Application	Reference(s)
Commercial kits
LIVE/ DEAD Fixable Aqua dead cell stain					Thermo/Fisher	Flow cyt	18
IFN-γ ELISpot basic (HRP) kit					MabTech	ELISpot	18
Primary antibodies
CD44	Mouse	IM7	IgG2b, κ	Rat	BD Pharmingen	Flow cyt	19
IL-4	Bovine	CC303	IgG2a	Mouse	Bio-Rad	Flow cyt	19
IFN-γ	Bovine	CC302	IgG1	Mouse	Bio-Rad	Flow cyt	19
IFN-γ	Mouse	XMG1.2	IgG1, κ	Rat	BD Pharmingen	Flow cyt	19
TNF	Mouse	MP6-XT22	IgG1	Rat	BD Pharmingen	Flow cyt	19
Thy1.1	Rat	OX-7	IgG1, κ	Mouse	BD Pharmingen	Flow cyt	19
CD11b	Mouse/human	M1/70	IgG2b, κ	Rat	BD Pharmingen or BioLegend	Flow cyt	18, 19
CD8a	Human	OK-T8	IgG2a	Mouse	eBioscience/Tonbo	Flow cyt	18, 19
CD4	Ferret	02	IgG1	Mouse	Sino Biological	Flow cyt	18, 19
MHC-II	Human	L243	IgG2a, κ	Mouse	BioLegend	Flow cyt	18
IgA, IgM, IgG	Ferret		Poly	Goat	LSBio	Flow cyt	18
CD59	Mouse	AL-21	IgM, κ	Rat	BD Pharmingen	Flow cyt	18
CD79a	Human	HM47	IgG1, κ	Mouse	eBioscience	Flow cyt	18
CD20	Ferret	71	IgG	Rabbit	Sino Biological	Flow cyt	18
CD3	Human	IS5033	Poly	Rabbit	Dako	IHC	44
Lysozyme	Human	A0099	Poly	Rabbit	Dako	IHC	44
CD20	Human	RB-9013-P	Poly	Rabbit	Thermo (Fisher)	IHC	44
CD79a	Human	HM57	IgG1, κ	Mouse	Dako	IHC	44
MHC-II	Human	TAL 1B5	IgG1, κ	Mouse	Dako	IHC	44
CD3	Human	PC3/188A	IgG1, κ	Mouse	Santa Cruz Biotech	Flow cyt	45
IFN-γ (capture Ab)	Cow	CC302	IgG1	Mouse	Bio-Rad	ELISpot/flow cyt	45
IFN-γ biotinylated (detection Ab)	Dog		Poly	Goat	R&D Systems		45, 46

^aAbbreviations: HRP, horseradish peroxidase conjugate; TNF, tumor necrosis factor; Ab, antibody; Flow cyt, flow cytometry; IHC, immunohistochemistry.