Table 1.

Principal Characteristics of Different cDNA Synthesis and Amplification Approaches for Microorganisms

	Strand Specificity	Positional Bias	Applied Cell Types	Transcript Coverage	Key References
Template switching	no	weakly 3′ or 5′-only	eukaryotic	yes or 5′-only	(31, 32, 45)
RCA	no	no	eukaryotic and prokaryotic	yes	(23, 24)
Ribo-SPIA	no	no	eukaryotic and prokaryotic	yes	(25, 26)