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. 2018 Jul 2;2018:2025914. doi: 10.1155/2018/2025914

Figure 7.

Figure 7

DAU reduced oxidative stress and ER stress of N2a/APP cells. (a) 8-OHdG (in green) immunostaining of N2a/WT and N2a/APP cells. DAPI (in blue) stained the cell nuclei. Proteins PRDX4 (b), PDIA1 (c), GRP75 (d), GRP78 (e), p-PERK (f), p-eIF2α, eIF2α (g), ATF-4 (h), and CHOP (i) in N2a/WT and N2a/APP cells were determined by Western blot analyses. β-Actin was used as a loading control for PRDX4, GRP78, GRP75, and PDIA1. α-Tubulin was used as a loading control for ATF-4, CHOP, and p-PERK. Total eIF2α was used as a loading control for p-eIF2α. N = 3. Data show the mean ± SEM. % P = 0.0052, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared to N2a/WT cells. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared to untreated N2a/APP.